Human FGFR2 beta (IIIb) Protein
Catalog No: FGFR2-HP004
- Species
- Human
- Expression System
- HEK293
- Tag
- His
- Activity
- Activity verified
Product overview
Recombinant Human FGFR2 beta (IIIb) Protein is expressed in HEK293 cells with a His tag at the C-terminus. It contains amino acid residues Arg152-Glu378 (UniProt accession: P21802-3).
Product Details
- Molecular Aliases
- Fibroblast growth factor receptor 2;FGFR-2;KGFR;K-sam;Keratinocyte growth factor receptor;CD332;BEK;KSAM
- Protein Length
- Arg152-Glu378
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 26.5 kDa. Due to glycosylation, the protein migrates to 50-65 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 0.1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 24 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Human FGFR2 beta (IIIb) on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.
The purity of Human FGFR2 beta (IIIb) is greater than 95% as determined by SEC-HPLC.
Immobilized Human FGFR2 beta (IIIb), His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Anti-FGFR2 Antibody, hFc Tag with the EC50 of 5.2ng/ml determined by ELISA (QC Test).
Serial dilutions of Anti-FGFR2 Antibody were added into Human FGFR2 beta (lllb), His Tag: Biotinylated Human FGF10, No Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 1.74ng/ml.
Anti-FGFR2 Antibody, hFc Tag can bind Human FGFR2 beta (IIIb), His Tag with an affinity constant of 1.98nM as determined in a SPR assay (Biacore T200).
Background
Four distinct genes encoding closely related FGF receptors, FGF R1 - 4, are known. All four genes for FGF Rs encode proteins with an N-terminal signal peptide, three immunoglobulin (Ig)-like domains, an acid-box region containing a run of acidic residues between the IgI and IgII domains, a transmembrane domain and the split tyrosine-kinase domain. Multiple forms of FGF R1 - 3 are generated by alternative splicing of the mRNAs. A frequent splicing event involving FGF R1 and 2 results in receptors containing all three Ig domains, referred to as the alpha isoform, or only IgII and IgIII, referred to as the beta isoform.
References
- Katoh, Masaru. FGFR inhibitors: Effects on cancer cells, tumor microenvironment and whole-body homeostasis (Review)[J]. International Journal of Molecular Medicine, 2016.
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