Human HLA-A*11:01&B2M&RASL11B (AVVGASGVGK) Monomer Protein
Catalog No: HLAA1101-HP003
- Species
- Human
- Expression System
- HEK293
- Tag
- His, Avi
Product overview
Recombinant Human HLA-A*11:01&B2M&RASL11B (AVVGASGVGK) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus. It contains Gly25-Thr305 (HLA-A*11:01), Ile21-Met119 (B2M) and AVVGASGVGK peptide (accession: AAV53343.1(HLA-A*11:01) & P61769(B2M) &AVVGASGVGK).
Product Details
- Molecular Aliases
- RASL11B; AVVGASGVGK; MHC
- Protein Length
- Gly25-Thr305
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 50.25 kDa. Due to glycosylation, the protein migrates to 52-65 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Supplied as 0.22 μm filtered solution in PBS (pH 7.4).
- State
- Liquid
- Storage Conditions
- Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Data Display
Human HLA-A*11:01&B2M&RASL11B (AVVGASGVGK) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human HLA-A*11:01&B2M&RASL11B (AVVGASGVGK) Monomer is greater than 95% as determined by SEC-HPLC.
Background
RAS (H-ras, K-ras, and N-ras), as the second largest mutated gene driver in various human cancers, has long been a vital research target for cancer. Its function is to transform the extracellular environment into a cascade of intracellular signal transduction. RAS mutant protein regulates tumor cell proliferation, apoptosis, metabolism and angiogenesis through downstream MAPK, PI3K and other signaling pathways.
References
- Chen S, Li F, Xu D, Hou K, Fang W, Li Y. The Function of RAS Mutation in Cancer and Advances in its Drug Research. Curr Pharm Des. 2019;25(10):1105-1114. doi: 10.2174/1381612825666190506122228. PMID: 31057104.
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