Human 2B4/CD244/SLAMF4 Protein
Catalog No: 2B4-HP003
- Species
- Human
- Expression System
- HEK293
- Tag
- an hFc (IgG1)
- Activity
- Activity verified
Product overview
Recombinant Human 2B4/CD244/SLAMF4 Protein is expressed in HEK293 cells with an hFc (IgG1) tag at the C-terminus. It contains amino acid residues Cys22-Arg221 (UniProt accession: Q9BZW8-2).
Product Details
- Molecular Aliases
- 2B4; CD244 antigen; CD244; h2B4; NAIL; NKR2B4; Nmrk; SLAMF4
- Protein Length
- Cys22-Arg221
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 49 kDa. Due to glycosylation, the protein migrates to 68-75 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 0.1 EU per ug by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in 20mM Tris, 150mM NaCl (pH 8.0). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Human 2B4 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human 2B4 is greater than 95% as determined by SEC-HPLC.
Immobilized Human CD48, His Tag (Cat. SLA-HM148) at 2μg/ml (100μl/well) on the plate. Dose response curve for Human 2B4, hFc Tag with the EC50 of 90.1ng/ml determined by ELISA. (QC Test)
Loaded Human CD48, His Tag on Anti-His-Biosensor can bind Human 2B4, hFc Tag with an affinity constant of 5.79 nM as determined in BLI assay.
Background
2B4 (CD244) is expressed by memory-phenotype CD8(+) T cells and all natural killer (NK) cells. The ligand for 2B4, CD48, is expressed on hematopoietic cells. 2B4 is conserved in humans and mice, and a number of reports have linked 2B4 with activation of lymphocytes.Engagement of 2B4 on NK cell surfaces with specific antibodies or CD48 can trigger cell mediated cytotoxicity, interferon γ secretion, phosphoinositol turnover and NK cell invasiveness.
References
- Velikovsky C A, Deng L, Chlewicki L K, et al. Structure of Natural Killer Receptor 2B4 Bound to CD48 Reveals Basis for Heterophilic Recognition in Signaling Lymphocyte Activation Molecule Family[J]. Immunity, 2007, 27(4):0-584. Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.
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