Biotinylated Mouse H-2K(b)&B2M&OVA (SIINFEKL) Monomer Protein, Ultra Low Endotoxin
Catalog No: H2KB-HB002
- Species
- Mouse
- Expression System
- HEK293
- Tag
- His, Avi
Product overview
Recombinant Biotinylated Mouse H-2K(b)&B2M&OVA (SIINFEKL) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus. It contains His24-Pro297 (H-2K(b)), Ile21-Met119 (B2M) and SIINFEKL peptide (accession: P01901(H-2K(b)) & P01887(B2M) &SIINFEKL).
Product Details
- Molecular Aliases
- H-2K; OVA; MHC; H2-K1;
- Protein Length
- His24-Pro297
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 50.20 kDa. Due to glycosylation, the protein migrates to 52-70 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 0.01 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Mouse H-2K(b)&B2M&OVA (SIINFEKL) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Mouse H-2K(b)&B2M&OVA (SIINFEKL) Monomer Protein is greater than 95% as determined by SEC-HPLC.
Background
Ovalbumin (OVA) has been historically a popular source of such antigens, since OVA can induce both humoral and cellular immune responses based on well-characterised peptide epitopes. The OVA257-264 octapeptide was one of the frst OVA epitopes to be characterised, it has an amino acid sequence SIINFEKL, which is recognised by cytotoxic T lymphocytes. SIINFEKL forms fbrillar assemblies similar to other peptide hydrogels. Te immunoactive properties of this peptide can therefore be related to its self-assembling nature.
References
- Kamalov M, Kählig H, Rentenberger C, Müllner ARM, Peterlik H, Becker CFW. Ovalbumin Epitope SIINFEKL Self-Assembles into a Supramolecular Hydrogel. Sci Rep. 2019 Feb 25;9(1):2696. doi: 10.1038/s41598-019-39148-8. PMID: 30804439; PMCID: PMC6390181.
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