Biotinylated Mouse APLN Protein
Catalog No: APLN-MB001
- Species
- Mouse
- Expression System
- HEK293
- Tag
- an hFc (IgG1), Avi
Product overview
Recombinant Biotinylated Mouse APLN Protein is expressed in HEK293 cells with an hFc (IgG1) tag and Avi tag at the C-terminus. It contains amino acid residues Val23-Phe77 (UniProt accession: Q9R0R4).
Product Details
- Molecular Aliases
- Apelin;APJ endogenous ligand;APEL
- Protein Length
- Val23-Phe77
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 34.81 kDa. Due to enzyme lysis and glycosylation, the protein migrates to 38-48 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS, 0.2M Arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Mouse APLN on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Background
Macrophages play key roles during cardiovascular diseases (CVD) and their related complications.Apelin (APLN) is a key molecule, whose roles during CVD have been documented previously. Therefore, it has been hypothesized that APLN may perform its roles via modulation of macrophages.
References
- Arababadi MK, Asadikaram P, Asadikaram G. APLN/APJ pathway: The key regulator of macrophage functions. Life Sci. 2019 Sep 1;232:116645. doi: 10.1016/j.lfs.2019.116645. Epub 2019 Jul
- PMID: 31299236.
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