Rhesus macaque LILRB1/CD85j/ILT2 Protein
Catalog No: LILRB1-CP006
- Species
- Rhesus macaque
- Expression System
- HEK293
- Tag
- an hFc (IgG1)
- Activity
- Activity verified
Product overview
Recombinant Rhesus macaque LILRB1/CD85j/ILT2 Protein is expressed in HEK293 cells with an hFc (IgG1) tag at the C-terminus. It contains amino acid residues Met1-His474 (accession: NP_001035762.2).
Product Details
- Molecular Aliases
- ILT2; ILT-2; ILT2FLJ37515; LILRB1; LIR1; MIR7; CD85J; XXbac-BCX85G21.4
- Protein Length
- Met1-His474
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 75.2 kDa. Due to glycosylation, the protein migrates to 80-90 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Rhesus macaque LILRB1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Rhesus macaque LILRB1 is greater than 95% as determined by SEC-HPLC.
Immobilized Rhesus macaque HLA-G&B2M&Peptide (RIIPRHLQL) Tetramer, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Rhesus macaque LILRB1, hFc Tag with the EC50 of 0.17μg/ml determined by ELISA (QC Test).
Rhesus macaque LILRB1, hFc Tag captured on CM5 Chip via Protein A can bind Rhesus macaque HLA-G&B2M&Peptide (RIIPRHLQL) Tetramer, His Tag with an affinity constant of 1.74 nM as determined in SPR assay (Biacore T200).
Anti-LILRB1 Antibody immobilized on CM5 Chip can bind Rhesus macaque LILRB1, hFc Tag with an affinity constant of 47.69 nM as determined in SPR assay (Biacore T200).
Background
LILRB1, also known as CD85j and ILT2, is a 110 kDa transmembrane glycoprotein in the LILR immunoregulatory protein family. Mature human LILRB1 consists of a 438 amino acid (aa) extracellular domain (ECD) with 4 tandem Ig-like domains, a 21 aa transmembrane segment, and a 168 aa cytoplasmic domain with 4 inhibitory ITIM motifs.LILRB1 is a receptor for class I MHC antigens. Recognizes a broad spectrum of HLA-A, HLA-B, HLA-C, HLA-G and HLA-F alleles. Receptor for H301/UL18, a human cytomegalovirus class I MHC homolog. Ligand binding results in inhibitory signals and down-regulation of the immune response.
References
- Apps R, Gardner L, Sharkey A, et al. A homodimeric complex of HLA-G on normal trophoblast cells modulates antigen-presenting cells via LILRB1 [J]. European Journal of Immunology, 2007, 37(7):1924-1937. Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.
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