Human/Cynomolgus/Rhesus macaque CD28 Protein
Catalog No: CD28-HP002
- Species
- Human/Cyno/Rhesus
- Expression System
- HEK293
- Tag
- an hFc (IgG1), Flag
- Activity
- Activity verified
Product overview
Recombinant Human/Cynomolgus/Rhesus macaque CD28 Protein is expressed in HEK293 cells with an hFc (IgG1) tag and Flag tag at the N-terminus. It contains amino acid residues Asn19-Pro152 (UniProt accession: P10747-1).
Product Details
- Molecular Aliases
- CD28 antigen (Tp44); CD28 molecule; CD28; MGC138290
- Protein Length
- Asn19-Pro152
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 42.6 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Human/Cynomolgus/Rhesus macaque CD28 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human/Cynomolgus/Rhesus macaque CD28 was greater than 95% as determined by SEC-HPLC.
Immobilized Human/Cynomolgus/Rhesus macaque CD28, hFc Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-CD28 Antibody, hFc Tag with the EC50 of 27.2ng/ml determined by ELISA (QC Test).
Background
CD28 is the receptor for CD80 (B7-1) and CD86 (B7-2) proteins.When activated by Toll-like receptor ligands, the CD80 expression is upregulated in antigen presenting cells (APCs). The CD86 expression on antigen presenting cells is constitutive. CD28 is the only B7 receptor constitutively expressed on naive T cells.
References
- Suntharalingam G, Perry M R, Ward S, et al. Cytokine Storm in a Phase 1 Trial of the Anti-CD28 Monoclonal Antibody TGN1412[J]. New England Journal of Medicine, 2006, 355(10):1018-1028. Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.
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