Cynomolgus/Rhesus macaque 4-1BB/TNFRSF9 Protein, Ultra Low Endotoxin
Catalog No: 41BB-CP002
- Species
- Cyno/Rhesus
- Expression System
- HEK293
- Tag
- His
- Activity
- Activity verified
Product overview
Recombinant Cynomolgus/Rhesus macaque 4-1BB/TNFRSF9 Protein is expressed in HEK293 cells with a His tag at the C-terminus. It contains amino acid residues Leu24-Gln186 (accession: XP_005544945.1).
Product Details
- Molecular Aliases
- CD137; TNFRSF9; 4-1BB ligand receptor; 41BB; 4-1BB; CDw137; FLJ43501; ILA; T cell antigen ILA
- Protein Length
- Leu24-Gln186
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 18.4 kDa. Due to glycosylation, the protein migrates to 30-40 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 0.01 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Cynomolgus/Rhesus macaque 4-1BB on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Cynomolgus/Rhesus macaque 4-1BB is greater than 95% as determined by SEC-HPLC.
Immobilized Cynomolgus/Rhesus macaque 4-1BB, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Cynomolgus 4-1BB Ligand, hFc Tag with the EC50 of 2.9ng/ml determined by ELISA (QC Test).
Loaded Cynomolgus 4-1BB Ligand, hFc Tag on ProA-Biosensor can bind Cynomolgus/Rhesus macaque 4-1BB, His Tag with an affinity constant of 23.5 nM as determined in BLI assay.
Background
4-1BB, is also known as CD137, is a type 2 transmembrane glycoprotein receptor belonging to the TNF superfamily.CD137 can be expressed by activated T cells, but to a larger extent on CD8 than on CD4 T cells. In addition, CD137 expression is found on dendritic cells, B cells, follicular dendritic cells, natural killer cells, granulocytes and cells of blood vessel walls at sites of inflammation.
References
- Vinay D S, Kwon B S. 4-1BB (CD137), an inducible costimulatory receptor, as a specific target for cancer therapy[J]. BMB Reports, 2014, 47(3):122-129. Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.
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