Biotinylated Human HLA-A*02:01&B2M&GSTO-2 (LLWPWFERL) Monomer Protein
Catalog No: HLAA0201-HB002
- Species
- Human
- Expression System
- E. coli
- Tag
- His, Avi
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Human HLA-A*02:01&B2M&GSTO-2 (LLWPWFERL) Monomer Protein is expressed from E.coli with His tag and Avi tag at the C-terminus. It contains Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and LLWPWFERL peptide (accession: A0A140T913(HLA-A*02:01) & P61769(B2M) &LLWPWFERL).
Product Details
- Molecular Aliases
- LLWPWFERL; GSTO-2; GSTO 2-2; MMA(V) reductase; GSTO2
- Protein Length
- Gly25-Thr305
- Expression System
- E. coli
- Theoretical Molecular Weight
- The protein has a predicted MW of 35.6 kDa (HLA-A*02:01) and 11.9 kDa (B2M) same as Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Supplied as 0.22 μm filtered solution in 20 mM Tris, 200 mM NaCl (pH 8.0).
- State
- Liquid
- Storage Conditions
- Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Data Display
Biotinylated Human HLA-A*02:01&B2M&GSTO-2 (LLWPWFERL) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human HLA-A*02:01&B2M&GSTO-2 (LLWPWFERL) Monomer is greater than 95% as determined by SEC-HPLC.
Immobilized Biotinylated Human HLA-A*02:01&B2M&GSTO-2 (LLWPWFERL) Monomer, His Tag at 2 μg/ml (100 μl/well) on the streptavidin precoated plate (5 μg/ml). Dose response curve for Anti-B2M Antibody, mFc Tag with the EC50 of 12.2 ng/ml determined by ELISA.
Background
Glutathione S-transferase omega-2 (GSTO2) is a unique member of the glutathione transferase superfamily with potential roles in cell growth regulation and protection against oxidative stress. It is distinct due to its lack of traditional GST activity, and it may contribute to disease development through post-transcriptional mechanisms.
References
- [1] Board PG, Menon D. Structure, function and disease relevance of Omega-class glutathione transferases. Arch Toxicol. 2016 May;90(5):1049-67. doi: 10.1007/s00204-016-1691-1.
- [2] Terayama M, Yamada K, Hagiwara T, Inazuka F, Sezaki T, Igari T, Yokoi C, Nohara K, Soma D, Dohi T, Kawamura YI. Glutathione S-transferase omega 2 regulates cell growth and the expression of E-cadherin via post-transcriptional down-regulation of β-catenin in human esophageal squamous cells. Carcinogenesis. 2020 Jul 14;41(7):875-886. doi: 10.1093/carcin/bgz189.
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