Biotinylated Human ANGPTL2/Angiopoietin-like 2 Protein
Catalog No: ANGPTL2-HB001
- Species
- Human
- Expression System
- HEK293
- Tag
- His, Avi
Product overview
Recombinant Biotinylated Human ANGPTL2/Angiopoietin-like 2 Protein is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus. It contains amino acid residues Thr260-His493 (UniProt accession: Q9UKU9-1).
Product Details
- Molecular Aliases
- ARP2; HARP; angiopoietin-like 2; ANGPTL2; ANGRP2; MGC8889
- Protein Length
- Thr260-His493
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 30.1 kDa. Due to glycosylation, the protein migrates to 31-35 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS, 200mM L-arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Human ANGPTL2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human ANGPTL2 is greater than 95% as determined by SEC-HPLC.
Background
Angiopoietin-like 2 (ANGPTL2) is a proinflammatory protein belonging to the angiopoietin-like family. ANGPTL2 is secreted and detected in the systemic circulation. Different observational clinical studies reported that circulating levels of ANGPTL2 increase significantly in various chronic inflammatory diseases and showed associations between ANGPTL2 levels and diagnosis and/or prognosis of cardiovascular diseases, diabetes, chronic kidney disease, and various types of cancers.
References
- Thorin-Trescases N, Thorin E. High Circulating Levels of ANGPTL2: Beyond a Clinical Marker of Systemic Inflammation. Oxid Med Cell Longev. 2017;2017:1096385. doi: 10.1155/2017/1096385. Epub 2017 Aug
- PMID: 29138671; PMCID: PMC5613648. Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.
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