Non-biotinylated Mouse OX40/TNFRSF4/CD134 Protein
Catalog No: OX40-MP001
- Species
- Mouse
- Expression System
- HEK293
- Tag
- His, Avi
- Activity
- Activity verified
Product overview
Recombinant Mouse OX40/TNFRSF4/CD134 Protein is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus. It contains amino acid residues Val20-Pro211 (UniProt accession: P47741).
Product Details
- Molecular Aliases
- CD134; OX40; OX40L receptor; TNFRSF4; ACT-135; Ly-70; OX40 homologue; TXGP1L; IMD16
- Protein Length
- Val20-Pro211
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 24.2 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 90% as determined by HPLC
- Endotoxin
- Less than 1 EU per ug by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Mouse OX40 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Mouse OX40 is greater than 90% as determined by SEC-HPLC.
Immobilized Mouse OX40 Ligand, hFc Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Mouse OX40, His Tag with the EC50 of 0.91μg/ml determined by ELISA. (QC Test)
Background
Tumor necrosis factor receptor superfamily, member 4 (TNFRSF4), also known as CD134 and OX40 receptor. OX40 is a secondary co-stimulatory immune checkpoint molecule, expressed after 24 to 72 hours following activation; its ligand, OX40L, is also not expressed on resting antigen presenting cells, but is following their activation.
References
- Al-Shamkhani A, Mallett S, Brown M H, et al. Affinity and Kinetics of the Interaction between Soluble Trimeric OX40 Ligand, a Member of the Tumor Necrosis Factor Superfamily, and Its Receptor OX40 on Activated T Cells[J]. Journal of Biological Chemistry, 1997, 272(8):5275-5282. Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.
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