Human HLA-A*02:01&B2M&HKDC1 (KVDRFLYHM) Monomer Protein
Catalog No: HLAA0201-HP002
- Species
- Human
- Expression System
- E. coli
- Tag
- His, Avi
- Activity
- Activity verified
Product overview
Recombinant Human HLA-A*02:01&B2M&HKDC1 (KVDRFLYHM) Monomer Protein is expressed from E.coli with His tag and Avi tag at the C-terminus. It contains Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and KVDRFLYHM peptide (accession: A0A140T913(HLA-A*02:01) & P61769(B2M) &KVDRFLYHM).
Product Details
- Molecular Aliases
- KVDRFLYHM; HKDC1; EC:2.7.1.1
- Protein Length
- Gly25-Thr305
- Expression System
- E. coli
- Theoretical Molecular Weight
- The protein has a predicted MW of 35.6 kDa (HLA-A*02:01) and 11.9 kDa (B2M) same as Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Supplied as 0.22 μm filtered solution in 20mM Tris, 200mM NaCl (pH 8.0).
- State
- Liquid
- Storage Conditions
- Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Data Display
Human HLA-A*02:01&B2M&HKDC1 (KVDRFLYHM) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human HLA-A*02:01&B2M&HKDC1 (KVDRFLYHM) Monomer is greater than 95% as determined by SEC-HPLC.
Immobilized Human HLA-A*02:01&B2M&HKDC1 (KVDRFLYHM) Monomer, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-B2M Antibody, mFc Tag with the EC50 of 16.6ng/ml determined by ELISA. (QC Test)
Background
Hexokinase (HK) is an enzyme that irreversibly phosphorylates hexoses (six-carbon sugars), forming hexose phosphate. In most organisms, glucose is the most important substrate for hexokinases, and glucose-6-phosphate is the most important product. There are four known isoforms of hexokinase in humans: HK1, HK2, HK3, and HK4 (also known as glucokinase). Each isoform has distinct properties and functions within the cell.
References
- [1] Baik SH, Ramanujan VK, Becker C, Fett S, Underhill DM, Wolf AJ. Hexokinase dissociation from mitochondria promotes oligomerization of VDAC that facilitates NLRP3 inflammasome assembly and activation. Sci Immunol. 2023 Jun 23;8(84):eade7652. doi: 10.1126/sciimmunol.ade7652. Epub 2023 Jun 16. PMID: 37327321; PMCID: PMC10360408.
- [2] Guo D, Meng Y, Jiang X, Lu Z. Hexokinases in cancer and other pathologies. Cell Insight. 2023 Jan 3;2(1):100077. doi: 10.1016/j.cellin.2023.100077. PMID: 37192912; PMCID: PMC10120283.
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