Biotinylated Cynomolgus Fc gamma RIII/CD16 Protein
Catalog No: CD16-CB001
- Species
- Cynomolgus
- Expression System
- HEK293
- Tag
- His, Avi
- Activity
- Activity verified
Product overview
Recombinant Biotinylated Cynomolgus Fc gamma RIII/CD16 Protein is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus. It contains amino acid residues Gly17-Gln208 (UniProt accession: Q8SPW2-1).
Product Details
- Molecular Aliases
- IgG Fc receptor III; Fc-gamma RIII; FcRIII; FCGR3; CD16; CD16A; FCG3; FcgRIII; FCR-10; FCRIIIA; IGFR3; IMD20; FcγRIII; Fc γRIII; Fcγ RIII; Fc γ RIII
- Protein Length
- Gly17-Gln208
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 24.86 kDa. Due to glycosylation, the protein migrates to 42-52 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Cynomolgus Fc gamma RIII on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Cynomolgus Fc gamma RIII is greater than 95% as determined by SEC-HPLC.
Loaded Biotinylated Cynomolgus Fc gamma RIII, His-Avi tag on Anti-His-Biosensor can bind Rituximab, hFc Tag with an affinity constant of 0.17 µM as determined in BLI assay. (QC Test)
Biotinylated Cynomolgus Fc gamma RIII, His Tag captured on CM5 Chip via Anti-His Antibody can bind Rituximab with an affinity constant of 0.15 μM as determined in SPR assay (Biacore T200).
Background
Immunoglobulin G (IgG) Fc receptors play a critical role in linking IgG antibody-mediated immune responses with cellular effector functions. A high resolution map of the binding site on human IgG1 for human Fc gamma RI, Fc gamma RIIA, Fc gamma RIIB, Fc gamma RIIIA, and FcRn receptors has been determined.A common set of IgG1 residues is involved in binding to all Fc gamma R; Fc gamma RII and Fc gamma RIII also utilize residues outside this common set.
References
- Shields RL, Namenuk AK, Hong K, Meng YG, Rae J, Briggs J, Xie D, Lai J, Stadlen A, Li B, Fox JA, Presta LG. High resolution mapping of the binding site on human IgG1 for Fc gamma RI, Fc gamma RII, Fc gamma RIII, and FcRn and design of IgG1 variants with improved binding to the Fc gamma R. J Biol Chem. 2001 Mar 2;276(9):6591-604. doi: 10.1074/jbc.M009483200. Epub 2000 Nov
- PMID: 11096108.
Have questions about this product?
Our technical team can help with product selection, application questions and order support.