Mouse Fc gamma RIV/CD16-2 Protein
Catalog No: CD162-MP001
- Species
- Mouse
- Expression System
- HEK293
- Tag
- His
- Activity
- Activity verified
Product overview
Recombinant Mouse Fc gamma RIV/CD16-2 Protein is expressed in HEK293 cells with a His tag at the C-terminus. It contains amino acid residues Gly21-Gln203 (UniProt accession: A0A0B4J1G0-1).
Product Details
- Molecular Aliases
- CD16-2; FcgammaRIV; Fcgr4; FcγRIV
- Protein Length
- Gly21-Gln203
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 22 kDa. Due to glycosylation, the protein migrates to 32-43 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Mouse Fc gamma RIV on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Mouse Fc gamma RIV is greater than 95% as determined by SEC-HPLC.
Mouse Fc gamma RIV, His Tag captured on CM5 Chip via anti-his antibody can bind Anti-CD3 Antibody with an affinity constant of 146 nM as determined in SPR assay (Biacore T200).
Loaded Mouse Fc gamma RIV, His Tag on Anti-His-Biosensor, can bind Anti-CD3 Antibody(OKT3) with an affinity constant of 0.21 µM as determined in BLI assay (Gator).
Background
FcgammaRIV is a relatively new IgG Fc receptor (FcgammaR) that is reported to contribute to the pathogenesis of autoimmune diseases.FcgammaRIII and FcgammaRIV are each essential to trigger an FcRgamma-linker for activation of T-cell-dependent signal that drives C5a production in the Arthus reaction. A combined requirement for FcgammaRIII and FcgammaRIV in autoimmune injury, and identify the linker for activation of T cells adaptor as an integral component of linked FcgammaR and C5a anaphylatoxin receptor activation to generate inflammation.
References
- Syed SN, Konrad S, Wiege K, Nieswandt B, Nimmerjahn F, Schmidt RE, Gessner JE. Both FcgammaRIV and FcgammaRIII are essential receptors mediating type II and type III autoimmune responses via FcRgamma-LAT-dependent generation of C5a. Eur J Immunol. 2009 Dec;39(12):3343-56. doi: 10.1002/eji.200939884. PMID: 19795417.
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