Biotinylated Human HLA-A*03:01&B2M&Influenza NP (ILRGSVAHK) Monomer Protein
Catalog No: HLAA0301-HB002
- Species
- Human
- Expression System
- E. coli
- Tag
- His, Avi
Product overview
Recombinant Biotinylated Human HLA-A*03:01&B2M&Influenza NP (ILRGSVAHK) Monomer Protein is expressed from E.coli with His tag and Avi tag at the C-terminus. It contains Gly25-Thr305(HLA-A*03:01), Ile21-Met119(B2M) and ILRGSVAHK peptide (accession: NP_002107.3(HLA-A*03:01) & P61769(B2M) &ILRGSVAHK).
Product Details
- Molecular Aliases
- Influenza NP; ILRGSVAHK
- Protein Length
- Gly25-Thr305
- Expression System
- E. coli
- Theoretical Molecular Weight
- The protein has a predicted MW of 35.39 kDa (HLA-A*03:01) and 11.9 kDa (B2M) same as Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Supplied as 0.22 μm filtered solution in 20mM Tris, 200mM NaCl (pH 8.0).
- State
- Liquid
- Storage Conditions
- Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Data Display
Biotinylated Human HLA-A*03:01&B2M&Influenza NP (ILRGSVAHK) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human HLA-A*03:01&B2M&Influenza NP (ILRGSVAHK) Monomer is greater than 95% as determined by SEC-HPLC.
Background
Influenza A virus (IAV) is a respiratory pathogen that causes seasonal epidemics with significant mortality. Influenza virus genome encapsidation is essential for the formation of a helical viral ribonucleoprotein (vRNP) complex composed of nucleoproteins (NP), the trimeric polymerase, and the viral genome. The RNA, located at the interface of neighboring NP protomers, interacts with conserved residues previously described as essential for the NP-RNA interaction. The NP undergoes conformational changes to enable RNA binding and helix formation.
References
- (1) Petrich A, Dunsing V, Bobone S, Chiantia S. Influenza A M2 recruits M1 to the plasma membrane: A fluorescence fluctuation microscopy study. Biophys J. 2021 Dec 21;120(24):5478-5490. doi: 10.1016/j.bpj.2021.11.023. Epub 2021 Nov 19. PMID: 34808098; PMCID: PMC8715234. (2) Chenavier F, Estrozi LF, Teulon JM, Zarkadas E, Freslon LL, Pellequer JL, Ruigrok RWH, Schoehn G, Ballandras-Colas A, Crépin T. Cryo-EM structure of influenza helical nucleocapsid reveals NP-NP and NP-RNA interactions as a model for the genome encapsidation. Sci Adv. 2023 Dec 15;9(50):eadj9974. doi: 10.1126/sciadv.adj9974. Epub 2023 Dec 15. PMID: 38100595; PMCID: PMC10848707.
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