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Human HLA-A*02:01&B2M&GSTO-2 (LLWPWFERL) Monomer Protein

Catalog No: HLAA0201-HP003

Species
Human
Expression System
E. coli
Tag
His, Avi
Activity
Activity verified

Product overview

Recombinant Human HLA-A*02:01&B2M&GSTO-2 (LLWPWFERL) Monomer Protein is expressed from E.coli with His tag and Avi tag at the C-terminus. It contains Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and LLWPWFERL peptide (accession: A0A140T913(HLA-A*02:01) & P61769(B2M) &LLWPWFERL).

Product Details

Molecular Aliases
LLWPWFERL; GSTO-2; GSTO 2-2; MMA(V) reductase; GSTO2
Protein Length
Gly25-Thr305
Expression System
E. coli
Theoretical Molecular Weight
The protein has a predicted MW of 35.6 kDa (HLA-A*02:01) and 11.9 kDa (B2M) same as Bis-Tris PAGE result.
Purity
> 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
Endotoxin
Less than 1 EU per μg by the LAL method.
Buffer / Formulation
Supplied as 0.22 μm filtered solution in 20 mM Tris, 200 mM NaCl (pH 8.0).
State
Liquid
Storage Conditions
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Data Display

Bis-Tris PAGE
HLAA0201-HP003 Bis-Tris-PAGE-white result

Human HLA-A*02:01&B2M&GSTO-2 (LLWPWFERL) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC
HLAA0201-HP003 SEC-HPLC result

The purity of Human HLA-A*02:01&B2M&GSTO-2 (LLWPWFERL) Monomer is greater than 95% as determined by SEC-HPLC.

ELISA
HLAA0201-HP003 ELISA result

Immobilized Human HLA-A*02:01&B2M&GSTO-2 (LLWPWFERL) Monomer, His Tag at 0.5 μg/ml (100 μl/well) on the plate. Dose response curve for Anti-B2M Antibody, mFc Tag with the EC50 of 6.4 ng/ml determined by ELISA. (QC Test)

Background

Glutathione S-transferase omega-2 (GSTO2) is a unique member of the glutathione transferase superfamily with potential roles in cell growth regulation and protection against oxidative stress. It is distinct due to its lack of traditional GST activity, and it may contribute to disease development through post-transcriptional mechanisms.

References

  1. [1] Board PG, Menon D. Structure, function and disease relevance of Omega-class glutathione transferases. Arch Toxicol. 2016 May;90(5):1049-67. doi: 10.1007/s00204-016-1691-1.
  2. [2] Terayama M, Yamada K, Hagiwara T, Inazuka F, Sezaki T, Igari T, Yokoi C, Nohara K, Soma D, Dohi T, Kawamura YI. Glutathione S-transferase omega 2 regulates cell growth and the expression of E-cadherin via post-transcriptional down-regulation of β-catenin in human esophageal squamous cells. Carcinogenesis. 2020 Jul 14;41(7):875-886. doi: 10.1093/carcin/bgz189.

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