Biotinylated Human HLA-B*15:01&B2M&SARS-CoV-2 epitope (NQKLIANQF) Monomer Protein
Catalog No: SCOV2EPI-HB001
- Species
- Human
- Expression System
- HEK293
- Tag
- His, Avi
Product overview
Recombinant Biotinylated Human HLA-B*15:01&B2M&SARS-CoV-2 epitope (NQKLIANQF) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus. It contains Gly21-Thr301(HLA-B*15:01), Ile21-Met119(B2M) and NQKLIANQF peptide (accession: AAA53258.1(HLA-B*15:01) & P61769(B2M) &NQKLIANQF).
Product Details
- Molecular Aliases
- MHC; SARS-CoV-2 epitope;
- Protein Length
- Gly21-Thr301
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 50.50 kDa. Due to glycosylation, the protein migrates to 52-68 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Biotinylated Human HLA-B*15:01&B2M&SARS-CoV-2 epitope (NQKLIANQF) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human HLA-B*15:01&B2M&SARS-CoV-2 epitope (NQKLIANQF) Monomer is greater than 95% as determined by SEC-HPLC.
Background
HLA-B*15:01 is strongly associated with asymptomatic infection with SARS-CoV-2 and is likely to be involved in the mechanism underlying early viral clearance. T cells from pre-pandemic individuals carrying HLA-B*15:01 were reactive to the immunodominant SARS-CoV-2 S-derived peptide NQKLIANQF, and 100% of the reactive cells displayed memory phenotype.
References
- [1] Augusto DG, Yusufali T, Sabatino JJ Jr, Peyser ND, Murdolo LD, Butcher X, Murray V, Pae V, Sarvadhavabhatla S, Beltran F, Gill G, Lynch K, Yun C, Maguire C, Peluso MJ, Hoh R, Henrich TJ, Deeks SG, Davidson M, Lu S, Goldberg SA, Kelly JD, Martin JN, Viera-Green CA, Spellman SR, Langton DJ, Lee S, Marcus GM, Olgin JE, Pletcher MJ, Gras S, Maiers M, Hollenbach JA. A common allele of HLA mediates asymptomatic SARS-CoV-2 infection. medRxiv [Preprint]. 2022 Oct 12:2021.05.13.21257065.
- [2] Augusto DG, Hollenbach JA. HLA variation and antigen presentation in COVID-19 and SARS-CoV-2 infection. Curr Opin Immunol. 2022 Jun;76:102178.
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