Chimeric HLA-A*02:01 (mα3) &mB2M&MAGE-A2 (KMVELVHFL) Monomer Protein
Catalog No: MAGEA2-HP001
- Species
- Human&Mouse
- Expression System
- HEK293
- Tag
- His
Product overview
Recombinant Chimeric HLA-A*02:01 (mα3) &mB2M&MAGE-A2 (KMVELVHFL) Monomer Protein is expressed from HEK293 with His tag at the C-terminus. It contains Gly25-Thr206 (Human HLA-A*02:01 α1&α2) and Asp207-Glu299 (Mouse H-2Ld α3), Ile21-Met119 (mB2M) and KMVELVHFL peptide (accession: A0A140T913(Human HLA-A*02:01 α1 & α2) & P01897(Mouse H-2Ld α3) & P01887(Mouse B2M) &KMVELVHFL).
Product Details
- Molecular Aliases
- MAGE-A2;MAGEA2
- Protein Length
- Gly25-Thr206
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 48.00 kDa. Due to glycosylation, the protein migrates to 50-65 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Supplied as 0.22 μm filtered solution in PBS (pH 7.4).
- State
- Liquid
- Storage Conditions
- Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Data Display
Chimeric HLA-A*02:01 (mα3) &mB2M&MAGE-A2 (KMVELVHFL) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Chimeric HLA-A*02:01 (mα3) &mB2M&MAGE-A2 (KMVELVHFL) Monomer is greater than 95% as determined by SEC-HPLC.
Background
Melanoma antigen gene (MAGE)-A2 (MAGEA2) is a member of the MAGE-A family proteins widely studied for cancer vaccine development and identification of tumor markers. MAGEA2 plays an oncogenic role in glioma progression, and they provide insight into MAGEA2 application as a novel predictor of clinical outcomes and a potential glioma biomarker.
References
- Meng Q, Luo G, Liu B, Sun Y, Yan Z. Melanoma-associated antigen A2 is overexpressed in glioma and associated with poor prognosis in glioma patients. Neoplasma. 2018;65(4):604-609. doi: 10.4149/neo_2018_170625N440. PMID: 30064232.
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