Human B7-H5/Gi24/VISTA Protein
Catalog No: VISTA-HF001
- Species
- Human
- Expression System
- HEK293
- Tag
- an hFc (IgG1)
- Activity
- Activity verified
Product overview
Recombinant Human B7-H5/Gi24/VISTA Protein is expressed in HEK293 cells with an hFc (IgG1) tag at the C-terminus. It contains amino acid residues Phe33-Ala194 (UniProt accession: Q9H7M9).
Product Details
- Molecular Aliases
- B7H5; B7-H5; VISTA; VSIR; PD1H; PD-1H; 4632428N05Rik; C10orf54; Dies1; Gi24; PP2135; SISP1
- Protein Length
- Phe33-Ala194
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 44.9 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Human B7-H5 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human B7-H5 is greater than 95% as determined by SEC-HPLC
Immobilized Human B7-H5, hFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Anti-B7-H5 Antibody, hFc Tag with the EC50 of 8.4ng/ml determined by ELISA (QC Test).
Human B7-H5, hFc Tag captured on CM5 Chip via Protein A can bind Human VSIG3, His Tag with an affinity constant of 14.44 μM as determined in SPR assay (Biacore T200).
Background
B7-H5, also known as VISTA, B7H5, Dies1, SISP1 and C10orf54, is a 55-65 kDa member of the Ig superfamily. It is a transmembrane molecule expressed in bone, on embryonic stem cells (ESCs), and on tumor cell surfaces.
References
- Byers J T, Paniccia A, Kaplan J, et al. Expression of the Novel Costimulatory Molecule B7-H5 in Pancreatic Cancer[J]. Annals of Surgical Oncology,
- Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.
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