Non-biotinylated Human SIRP Beta/CD172b Protein
Catalog No: SIRPB-HB001
- Species
- Human
- Expression System
- HEK293
- Tag
- His, Avi
Product overview
Recombinant Human SIRP Beta/CD172b Protein is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus. It contains amino acid residues Glu30-Ala369 (UniProt accession: O00241-1).
Product Details
- Molecular Aliases
- SIRP-beta-1 isoform 3; SIRPB1; CD172b; DKFZp686A05192; FLJ26614; 9930027N05Rik; SIRP-beta; Sirpb; Sirpb1; Sirpb1a;SIRP-β-1 isoform 3; SIRP-β
- Protein Length
- Glu30-Ala369
- Expression System
- HEK293
- Theoretical Molecular Weight
- The protein has a predicted MW of 40 kDa. Due to glycosylation, the protein migrates to 52-60 kDa based on Bis-Tris PAGE result.
- Purity
- > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
- Endotoxin
- Less than 1 EU per μg by the LAL method.
- Buffer / Formulation
- Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
- State
- Lyophilized
- Storage Conditions
- -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Reconstitution Advice
- Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Data Display
Human SIRP Beta on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.
The purity of Human SIRP Beta is greater than 95% as determined by SEC-HPLC.
Background
SIRP beta 1 is a type I transmembrane protein belonging to the SIRP family within the Ig superfamily. Members of this family are characterized by an extracellular region containing a V-set Ig domain containing a J-like sequence and two C1-set Ig domains.SIRP beta 1 is immunoglobulin-like cell surface receptor involved in the negative regulation of receptor tyrosine kinase-coupled signaling processes. Participates also in the recruitment of tyrosine kinase SYK. Triggers activation of myeloid cells when associated with TYROBP.
References
- Takizawa H, Manz M G. Macrophage tolerance: CD47–SIRP-α–mediated signals matter[J]. Nature Immunology, 2007, 8(12):1287-1289. Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.
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